TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 7

Inhibition of necroptosis reduces the inflammatory response after IRI of NAFLD. (a) Immunoblot analysis of JNK, cJUN, ERK, IKBα, p38, and p65 of CD- and HFD-fed mice with or without IRI. (b) Immunoblot analysis of JNK, cJUN, ERK, IKBα, p38, and p65 of CD-fed mice after IRI with Nec-1 and GSK872 pretreatment. (c) Immunoblot analysis of JNK, cJUN, ERK, IKBα, p38, and p65 of CD-fed mice after IRI with Nec-1 and GSK872 pretreatment. (d) Immunoblot analysis of JNK, c-JUN, ERK, IKBα, p38, and p65 of hepatocytes with Nec-1 and GSK872 pretreatment. (e) Representative F4-80 immunohistochemistry of liver sections with IRI in CD- and HFD-fed mice. Scale bars, 100 μm. (f) Representative MPO immunohistochemistry of liver sections with IRI in CD- and HFD-fed mice. Scale bars, 100 μm.

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