Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice

Figure 6

Effects of SS31 on Drp1, Mfn1, Caspase1, and IL-1β protein expression in HK-2 cells exposed to HG. (a) IF analysis of Drp1 expression and mitochondrial morphology in HK-2 cells exposed to HG conditions and pretreated with SS31 or Mdivi1. (b) Western blot analysis of Mfn1 (upper panel), Drp1, Caspase1 (middle panel), and IL-1β (bottom panel) protein expression in HK-2 cells exposed to HG conditions and pretreated with SS31 or Mdivi1. (c–e) Densitometric analyses of the Western blotting results: Drp1 to β-actin (c), Mfn1 to β-actin (d), Caspase1 to β-actin (e), and IL-1β to β-actin (f). The data are presented as , vs. HG groups, vs. LG groups, .

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