miR-200c Modulates the Pathogenesis of Radiation-Induced Oral Mucositis

Figure 3

miR-200c regulates ROS generation and DNA repair in NHKs after irradiation. (a) NHK/Control and NHK/miR-200c- cells were exposed to 5 Gy IR and stained with DHE. Fluorescence intensity was quantitatively determined by flow cytometry. and vs. NHK/Control cells. (b) Western blotting was performed for p47 and GAPDH (internal control) in NHK/Control and NHK/miR-200c- cells at indicated time after being exposed to 5 Gy. (c) NHK/Control and NHK/miR-200c- cells were exposed to 5 Gy radiation and neutral comet assay was performed. Photos were taken at 0 and 10 h postirradiation, and DNA DSB was quantitated by DNA containing ratio of head/tail (). vs. NHK/Control cells. (d) NHK/Control and NHK/miR-200c- cells were irradiated at 5 Gy; cells were further stained with the γ-H2AX antibody 24 h after radiation. Representative images were taken; the percentage of γ-H2AX foci positive cells (>5 intranuclear foci) was statistically analyzed (). and vs. NHK/Control cells. (e) Western blotting was performed for γ-H2AX, p-p53, and GAPDH (internal control) in NHK/Control and NHK/miR-200c- cells at indicated time after being exposed to 5 Gy.

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