miR-200c Modulates the Pathogenesis of Radiation-Induced Oral Mucositis

Figure 4

miR-200c regulates proinflammatory cytokine induction and TGF-β/NF-κB activation in NHKs after irradiation. (a) NHK/Control and NHK/miR-200c- cells were exposed to 5 Gy IR; the expression of TNF-α, TGF-β, and IL-1α was measured by qPCR at 0, 3, 5, and 7 days postirradiation. , , and vs. NHK/Control cells. (b) NHK/Control and NHK/miR-200c- cells were exposed to 5 Gy radiation; the immunofluorescent images of NF-κB (phosphor S 536) were taken at 0, 15, 30, and 60 min postirradiation. The NF-κB nuclear translocated cell numbers were counted. (c) Western blotting was performed for NF-κB (phosphor S536), IκB (phosphor S32), IκB, and GAPDH (internal control) in NHK/Control and NHK/miR-200c- cells at indicated time after being exposed to 5 Gy. (d) Western blotting was performed for p-Smad2, Smad2, Smad7, and GAPDH (internal control) in NHK/Control and NHK/miR-200c- cells at indicated time after being exposed to 5 Gy.

(a)

(b)

(c)

(d)