MicroRNA-25 Protects Smooth Muscle Cells against Corticosterone-Induced Apoptosis

Figure 4

MOAP1 is a direct target of miR-25. MicroRNA-25 seed sequence and its complementary binding site in MOAP1 3-UTR are highlighted (a). VSMCs were untreated or transfected with pre-miR-25 or antagomiR-25 for 24 h or 48 h. Expression of MOAP1 mRNA was determined using qRT-PCR and western blot (b, c, and d). Luciferase activities of constructs containing the mutant 3-UTR segment of MOAP1 (e). Results are expressed as . PBS-24 and PBS-48 h: VSMCs were treated with PBS for 24 and 48 hours. Corti-24 h and Corti-48 h: VSMCs were treated with corticosterone for 24 and 48 hours; Crt-24 h, Crt-48 h, KD-24 h, KD-48 h, OE-24 h, and OE-48 h: VSMCs were infected with control vector, pre-miR-25, or antagomiR-25 for 24 and 48 hours; Crt: control vector; KD-miR-25: antagomiR-25; and OE-miR-25: pre-miR-25.

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