Hyperosmotic Stress Initiates AMPK-Independent Autophagy and AMPK- and Autophagy-Independent Depletion of Thioredoxin 1 and Glyoxalase 2 in HT22 Nerve Cells

Figure 3

Hyperosmotic stress modulates autophagy markers LC3 and p62 in HT22 cells. Cells were treated for the indicated times and the indicted NaCl concentrations. Representative blot images of p62 (a) showing the respective quantification (b) and representative image of LC3 (c). Quantification of LC3 in cells treated with 150 mM NaCl: (d) total (LC3 I + LC3 II) and (e) the ratio of lipidated (LC3 II, lower band) to unlipidated (LC3 I, upper band) forms of LC3. Values are expressed relative to actin obtained from at least 4 independent experiments. Statistical significance is presented as , , or .

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