Hyperosmotic Stress Initiates AMPK-Independent Autophagy and AMPK- and Autophagy-Independent Depletion of Thioredoxin 1 and Glyoxalase 2 in HT22 Nerve Cells

Figure 8

ATG5-independent depletion of Trx1 and Glo2 in HT22 cells. The cells were treated with the control or ATG5 siRNA for 48 hrs and thereafter treated with 150 mM NaCl (OS) for 6 or 18 h. Representative images (a) and respective quantification of Trx1 (b) and Glo2 (c). The effectiveness of the ATG5 siRNA in decreasing ATG5 protein has been previously reported [23]. Representative blot images of 3 independent experiments. Data from 6 h and 18 h were pooled and presented in graphs (b) and (c). Statistical significance is presented relative to the untreated control as .

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