ASK1 Mediates Apoptosis and Autophagy during oxLDL-CD36 Signaling in Senescent Endothelial Cells

Figure 3

oxLDL-stimulated human monocytes (THP-1 cells) had increased CD36 and induced autophagy. (a) Total ROS amounts significantly increased from an oxLDL concentration of 10 μg/mL. (b) Western blot analysis demonstrated that CD36 protein expression was enhanced to levels as high as oxLDL concentration in THP-1 cells. (c) CD36 mRNA levels were elevated in a time-dependent manner at an oxLDL concentration of 40 μg/mL, as demonstrated by using real-time PCR. (d) Immunocytochemistry demonstrated that LC3II-positive THP-1 cells were detected at 40 μg/mL oxLDL in a time-dependent manner. At 48 hours, LysoTracker was fully shown as being colocalized to LC3II. Results are expressed by the mean and standard deviation. Significance is presented as follows: .

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