ASK1 Mediates Apoptosis and Autophagy during oxLDL-CD36 Signaling in Senescent Endothelial Cells

Figure 4

ASK1 modulates autophagy by oxLDL-CD36 signaling in THP-1 cells. (a) Total amounts of ROS in THP-1 cells were not significantly changed by inhibiting ASK1 or by blocking CD36. (b) LC3II and LysoTracker were detected in THP-1 cells and increased in a time-dependent manner. In immunocytochemistry, CD36 and LC3II signals were diminished by inhibiting ASK1 and blocking CD36. (c) Autophagy assay showed that oxLDL led to autophagy, and autophagy increased by inhibiting ASK1, not by blocking CD36. (d) Apoptotic cell death was evaluated by observing cleaved caspase-3 activity. Activity was reduced by inhibiting ASK1 or by blocking CD36, but supplemented ASK1 in CD36-masked THP-1 cells increased cell death. (e) Western blot evaluated the changes in each protein level by blocking CD36 or by inhibiting ASK1. Normal: THP-1 without oxLDL; FA6-152: masking antibody for CD36; NQDI-1: ASK1 inhibitor. Results are expressed by the mean and standard deviation. Significance is presented as follows: .

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