Histone Acetyltransferase-Dependent Pathways Mediate Upregulation of NADPH Oxidase 5 in Human Macrophages under Inflammatory Conditions: A Potential Mechanism of Reactive Oxygen Species Overproduction in Atherosclerosis

Figure 4

IF localization of H3K27ac, H3K9ac, and Nox5 proteins in the area of infiltrated immune cells/Mac, fibrous cap, and lipid-rich core of a human carotid atherosclerotic plaque. Representative IF images (5x, 10x, and 20x magnification) of 5 μm thick adjacent frozen sections depicting positively stained cells (Alexa Fluor™ 594) for H3K27ac, H3K9ac, Nox5, CD45, and αSMA in a human carotid atherosclerotic plaque. Note the appearance of specific staining of proteins within fibrous cap, and lipid-rich core of atherosclerotic plaque, especially at a higher magnification (i.e., 20x).