Histone Acetyltransferase-Dependent Pathways Mediate Upregulation of NADPH Oxidase 5 in Human Macrophages under Inflammatory Conditions: A Potential Mechanism of Reactive Oxygen Species Overproduction in Atherosclerosis

Figure 8

Overexpression of p300 or HAT1 upregulates the promoter activity of human Nox5 gene in Mac. The RAW264.7 cells were transiently transfected with Nox5 gene promoter-luciferase reporter gene constructs in the presence of empty vector or p300/HAT1 expression vectors. Representative agarose gel electrophoresis showing the digestion products of the c1-c8 constructs (a). Schematic depiction of the 5-deletion mutants of the Nox5 gene promoter used in the cotransfection assays (b). Induction of luciferase level directed by the DNA regulatory elements derived from human Nox5 gene promoter in response to p300 or HAT1 overexpression in cultured Mac (c). ; . values were taken in relation to the corresponding empty vector control.

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