FOXO1 Overexpression Attenuates Tubulointerstitial Fibrosis and Apoptosis in Diabetic Kidneys by Ameliorating Oxidative Injury via TXNIP-TRX

Figure 2

Tissue-specific expression of the Pax2-Foxo1 transgene in transgenic (Tg) mice. (a) Piggy-Pax2-Foxo1 construct for the generation of Tg mice. Schematic representation of the construct containing the Pax2 promoter, CA-Foxo1, and GFP sequences. The positions of PCR primers used to detect the transgene are shown. (b) Genomic identification of Tg mice. Foxo1-Tg mice (T1–T9) showed a PCR-amplified 490 bp product that was undetected in non-Tg mice (N1–N2). (c) Western blot analysis of FOXO1 expression in mouse renal proximal tubule extracts of male non-Tg (N1-N3) and Tg (T1-T3) mice; β-actin was used as an internal control. (d) Immunohistochemistry staining of FOXO1 and relative staining intensity in male non-Tg and Tg mouse kidneys, using rabbit anti-FOXO1 polyclonal antibodies. Scale bars, 50 μm.

(a)

(b)

(c)

(d)