Role of Cav-1 in HIV-1 Tat-Induced Dysfunction of Tight Junctions and Aβ-Transferring Proteins

Figure 2

Cell viability assay. Effects of puromycin at 0, 0.5, 1.0, 1.5, 2.0, 2.5, or 3.0 μg/mL for 24 h on the viability of HBEC-5i cells were assessed by a CCK8 assay. Cell viability was affected over 1 μg/mL of puromycin (a). Cav-1 expression in HBEC-5i cells was silenced by transduction with lentiviral vectors of Cav-1 shRNA. Effects of HIV-1 Tat at 0, 0.5, 1.0, 1.5, 2.0, 2.5, or 3.0 μg/mL for 24 h on the viability of HBEC-5i cells silenced with Cav-1 shRNA were also assessed by a CCK8 assay. Cell viability was not affected by 1 μg/mL HIV-1 Tat for 24 h (b). Data are representative of three independent experiments. , , and versus control.

(a)

(b)