Suppression of Gut Bacterial Translocation Ameliorates Vascular Calcification through Inhibiting Toll-Like Receptor 9-Mediated BMP-2 Expression

Figure 1

Bacteria translocation in adenine-induced CRF rats. (a-b) The mesenteric lymph node and spleen from Ctrl, CRF, and CRF plus antibiotics (CRF + Anti) rats were grinded with PBS and cultured on blood agar at 37°C for 36 h, and the colony number per gram tissue was calculated. (c-d) The mesenteric lymph node weight/body weight and spleen weight/body weight of Ctrl, CRF, and CRF + Anti rats were calculated. (e) Chromogenic End-point TAL Kit detected serum LPS levels in Ctrl, CRF, and CRF + Anti rats. (f) Serum bacterial DNA of Ctrl, CRF, and CRF + Anti rats was extracted using the QIAamp DNA Mini Kit, and real-time PCR analyzed the 16S rDNA level. (g) Serum TNFα levels of Ctrl, CRF, and CRF + Anti rats were measured by ELISA. (h) Thoracic aorta mRNA was extracted from Ctrl, CRF, and CRF + Anti rats, and real-time PCR analyzed the mRNA levels of TLR4, TLR7, TLR8, and TLR9. ~12, vs. Ctrl, ^# vs. CRF.

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