NADPH Oxidase 4 Contributes to Myoblast Fusion and Skeletal Muscle Regeneration

Figure 1

Nox4 contributes to skeletal muscle regeneration. (a) H&E stained sections of TA muscles from WT and Nox4-KO mice at 0, 7, and 14 days after 50 μL of CTX (20 μM) was injected. Scale bar, 50 μm. (b) CSAs of regenerating myofibers were analyzed at 0, 7, and 14 days after CTX injection. ^#p < 0.001 (n = 5). (c) Nox4 mRNA levels in TA muscles of WT and KO mice during regeneration after CTX injury was determined by RT-qPCR, using 36B4 for normalization. ^#p < 0.001. The distribution of fiber sizes was analyzed at 7 days (d) and 14 days (e) after CTX injection. , , ^#p < 0.001 (n = 5).

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