Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro

Figure 6

AL inhibits apoptosis of hepatocytes and TLR4/MyD88/NF-κB signal pathway. (a, b) Apoptosis of primary mouse hepatocytes was assayed using flow cytometry. (c, d) Western blot-assisted analysis of Bcl-2, Bax, C-caspase 3, and β-actin. (e, f) Western blot-assisted analysis of TLR4, FADD, MyD88, TRAF6, p-IKKα/β, p-NF-κB p65 and GAPDH. Values represent deviation (SD) values (). , versus the control group; ^#, ^## versus the H/R group; ^&, ^&& versus the H/R+AL group; one-way ANOVA with Tukey test.

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