RNA Expression Profile and Alternative Splicing Signatures of Genistein-Treated Breeder Hens Revealed by Hepatic Transcriptomic Analysis

Figure 1

Effects of 40 mg/kg dietary genistein supplementation of LBB hens on differential gene expression and functional enrichment. (a) Number of differentially expressed genes (DEGs) (up/downregulated) in LBB hens after genistein supplementation at 40 mg/kg. (b) Levels 2 and 3 of enriched biological processes. (c) Number of DEGs enriched in GOs and pathways. (d) The top highest PAS score of enriched biological processes. PAS score represents the pathway activation strength value, served as the activation profiles of the signaling pathways based on the expression of individual genes. (e) Level 6 of enriched biological processes. (f) Protein-protein interaction analysis using DEGs of the CON vs. LGE groups. Circular nodes represent genes/proteins; rectangles represent KEGG pathways or GO biological process terms. The pathways are colored with a gradient from yellow to blue, in which yellow indicates a smaller value and blue indicates a larger value. GO biologic processes are colored red. In the fold change analysis, genes/proteins are colored red for upregulation or green for downregulation.

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