Prevention of Huntington’s Disease-Like Behavioral Deficits in R6/1 Mouse by Tolfenamic Acid Is Associated with Decreases in Mutant Huntingtin and Oxidative Stress

Figure 9

Effect of tolfenamic acid on the viability of PC12 cells cultured with 3-nitropropionic acid (3-NP). PC12 cells were preincubated with control small interfering RNA (siRNA) or Nrf2 siRNA (60 nM). After 24 h of incubation, the cells were treated with tolfenamic acid (5 and 10 μM) for 24 h and then with 3-NP (15 mM) for 8 h (a). PC12 cells were treated with tolfenamic acid (5 and 10 μM) with or without ML385 (5 μM) for 24 h and then with 3-NP (15 mM) for 8 h (b). PC12 cells were treated with tolfenamic acid (10 μM) with or without BSO (15 μM) for 24 h and then with 3-NP (15 mM) for 8 h (c). After treatment, cell survival was determined using the MTT assay. All of the results are expressed as the . ; and .

(a)

(b)

(c)