Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation

Figure 4

PCS extract recovers the decreased insulin-stimulated glucose uptake and expressions of p-Akt and pIRS1/2 in HepG2 cells. (a) HepG2 cells were treated with MGO (0.1–1 mM, 12 h of treatment) and then stimulated with 0.5 μM insulin for 30 min. The cells were harvested, and the expression of p-Akt was examined by western blotting. (b) Glucose uptake was measured using glucose uptake colorimetric assay kit. (c) The expression of p-Akt and pIRS1/2 was examined by western blotting. Cells were stimulated with 0.5 μM insulin for 30 min. A representative blot is shown. (d, e) The intensity of band was quantified using Image Lab software. (d) Quantification of p-Akt/actin from Figure 2(c). (e) Quantification of p-IRS/actin from Figure 2(c). Data are shown as (, independent experiments); . The figure is representative of three independent experiments. CON: control; MGO: methylglyoxal; INS: insulin.

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