Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation

Figure 5

Antiglycan effect of PCS extract on MGO-induced AGE formation. (a) BSA (5 mg/mL) was mixed with 1 mM of MGO in PBS (pH 7.4) in the presence or absence of PCS extracts with concentrations of 50, 100, 200, and 500 μg/mL or 1 mM AG and incubated at 37°C for 7 days. AGE levels were determined by measuring fluorescence at the excitation/emission wavelengths of 355/460 nm. Data are shown as (). (b) Serum was obtained and AGE levels were analyzed by western blotting. The representative blot is shown from three independent experiments. CON: control; MGO: methylglyoxal; MGO+PCS 200: methylglyoxal+PCS extract 200 mg/kg; MGO+PCS 500: methylglyoxal+PCS extract 500 mg/kg; MGO+AG: methylglyoxal+aminoguanidine. (c) Quantification data of (b). (d) Representative image of western blot of GLO1 in liver tissue. (e) Quantification data of (d); , .

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