Research Article
Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation
Figure 6
Effects of PCS extract on MGO-induced ROS production in HepG2 cells and the expression of antioxidant enzyme in liver tissue of MGO-administered mice. (a) HepG2 cells were pretreated with the indicated concentration of PCS extract (50–500 μg/mL), followed by treatment with MGO (1 mM) for 1 h. Fluorescent intensity was measured after staining with CM-H2DCFDA (10 μM, 30 min). Data are shown as (). (b) GPx activity in liver tissue was measured by GPx assay kit. Data are shown as (). (c) Protein expressions of SOD1, SOD2, and HO-1 were examined by western blotting. A representative blot is shown. (d–f) The intensity of bands was quantified using Image Lab software. Quantification of SOD1/actin (d), SOD2/actin (e), and HO-1/actin (f) from (c). Data are shown as . , , and . CON: control; MGO: methylglyoxal; MGO+PCS 200: methylglyoxal+PCS extract 200 mg/kg; MGO+PCS 500: methylglyoxal+PCS extract 500 mg/kg.
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