Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation

Figure 7

Effects of PCS extract on the expression of inflammatory signaling molecules and mRNA levels of IL-1β, TNF-α, and iNOS in MGO-administered mice. (a) Liver tissues were obtained, and the expression of pNF-κB p65, p-ERK, and p-p38 was examined by western blotting. A representative blot is shown from three independent experiments. (b–d) The intensity of bands was quantified using Image Lab software. Quantification of p-p38/p38 (b), p-ERK/ERK (c), and p-NF-κB/actin (d) from (a). Data are shown as . mRNA expression of IL-1β (e), TNF-α (f), and iNOS (g) was examined by qRT-PCR analysis. Data are shown as (). , , and . CON: control; MGO: methylglyoxal; MGO+PCS 200: methylglyoxal+PCS extract 200 mg/kg; MGO+PCS 500: methylglyoxal+PCS extract 500 mg/kg; MGO+AG: methylglyoxal+aminoguanidine.

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