DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-<i>κ</i>B Signaling

<div>miR-122 was downregulated in the oxidative stress model of human intestinal epithelial cell line HT-29. (a) CCK-8 analysis of cell viability after treatment of H<sub>2</sub>O<sub>2</sub> with different concentrations. (b–d) The protein levels of MDA, SOD, and ROS in the supernatant of culture medium under different concentrations of H<sub>2</sub>O<sub>2</sub> were examined by ELISA. (e, f) The relative levels of miR-122 and SBP1 mRNA in HT-29 cells were detected by qRT-PCR under different concentrations of H<sub>2</sub>O<sub>2</sub>. (g) Western blotting analysis of SBP1 expression in HT-29 cells with different concentrations of H<sub>2</sub>O<sub>2</sub>. (h) The prediction of the binding between miR-122 and SBP1 by TargetScan. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-<i>κ</i>B Signaling 

Oxidative Medicine and Cellular Longevity

DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling

Figure 3