DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling

Figure 4

miR-122 protected H₂O₂-induced oxidative stress partially by p65NF-κB signaling in vitro. (a–c) miR-122, SBP1 mRNA, and protein level in HT-29 cells after transfection with pre-miR-122. vs. si-NC. (d) The efficiency of si-SBP1. vs. si-NC. (e) The protein levels of ROS, MDA, 8-OHdG, IL-6, and IL-8 were examined by ELISA in normal, H₂O₂, pre-miR-122, si-SBP1, and both pre-miR-122 and si-SBP1-added H₂O₂ group. vs. normal; ^## vs. si-NC. (f) Lower levels of p-p65, p65NF-κB, and ICAM and higher levels of GPX1 and p-IκBα were found in the pre-miR-122 or si-SBP1-added H₂O₂ group than the H₂O₂ group. Meanwhile, cotreatment of pre-miR-122 and si-SBP1 could intensify these effects. , vs. normal; ^#, ^##, ^### vs. si-NC.

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