DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling

Figure 5

Pre-miR-122 or si-SBP1 treatment ameliorated TNBS-induced CD partially by p65NF-κB signaling. (a) The macroscopic appearance of the inflammatory colon in the control, TNBS, pre-miR-122-added TNBS, and si-SBP1-added TNBS groups. vs. control (ethanol); ^#, ^## vs. TNBS. (b) qRT-PCR analysis of SBP1 in the above groups. (c–e) DAI and histology scores in the above groups. vs. control; ^# vs. TNBS. (f) The protein levels of 8-OHdG, GSH, MDA, SOD, IL-6, and IL-8 were examined by ELISA in the above groups. vs. control (ethanol); ^## vs. TNBS. (g) Protein levels of p-p65, p65NF-κB, ICAM, GPX1, and p-IκBα by Western blotting analysis. vs. control; ^#, ^## vs. TNBS.

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