DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling

Figure 6

Inhibition of miR-122 promoted H₂O₂-induced oxidative stress in vitro. (a–c) miR-122, SBP1 mRNA, and protein level in HT-29 cells after transfection with the miR-122 inhibitor. vs. normal. (d) The protein levels of ROS, MDA, 8-OHdG, IL-6, and IL-8 were examined by ELISA in normal, H₂O₂, and miR-122 inhibitor-added H₂O₂ groups. vs. normal; ^## vs. miR-122 inhibitor NC. (e) Higher levels of p-p65, p65NF-κB, and ICAM and lower levels of GPX1 and p-IκBα were found in the miR-122 inhibitor-added H₂O₂ group than the H₂O₂ group. vs. normal, ^## vs. miR-122 inhibitor NC.

(a)

(b)

(c)

(d)

(e)