DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling

Figure 7

Inhibition of miR-122 aggravated TNBS-induced colitis in vivo. (a) The macroscopic appearance of the inflammatory colon in the control, TNBS, and miR-122 inhibitor-added TNBS groups. (b) qRT-PCR analysis of SBP1 in the above groups. (c–e) DAI and histology scores in the above groups. vs. control, ^## vs. TNBS. (f) The protein levels of 8-OHdG, GSH, MDA, SOD, IL-6, and IL-8 were examined by ELISA in the above groups. vs. control, ^## vs. TNBS. (g) Protein levels of p-p65, p65NF-κB, ICAM, GPX1, and p-IκBα by Western blotting analysis. vs. control, ^## vs. TNBS.

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