Metformin Ameliorates Testicular Damage in Male Mice with Streptozotocin-Induced Type 1 Diabetes through the PK2/PKR Pathway

Figure 5

Met reduced diabetes mellitus-induced apoptosis in the testes. (a) A typical image of apoptotic cells stained by TUNEL (; ). (b) Typical immunohistochemical staining of Bax (; ). (c) Typical immunohistochemical staining of Bcl-2 (; ). (d) Typical immunohistochemical staining of PCNA (; ). (e) Quantification of Bax mRNA expression. (f) Quantification of Bcl-2 mRNA expression. (g) Quantification of PCNA mRNA expression. (h) A typical image of Bax and Bcl-2 proteins. (i) Quantification of Bax expression. (j) Quantification of Bcl-2 expression. (k) Quantification of the ratio of Bax/Bcl-2 expression. per group. The values are presented as the . vs. the control group; ^# vs. the DM group.