Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite

Table 10

(a) Expression of DNMT1 in GPx1 knockdown splenic lymphocytes of pigs

DNMT1 mRNA
Time	Groups	Contents
48 h	P-M			0.111
	M-Se			0.280
	M-D1			0.443
	M-D2			0.497
	M-D3			0.235
	M-D4			0.304
	D1-SD1			0.493
	D2-SD2			0.791
	D3-SD3			0.246
	D4-SD4			0.452

(b) Expression of DNMT3a in GPx1 knockdown splenic lymphocytes of pigs

DNMT3a mRNA
Time	Groups	Contents
48 h	P-M			0.770
	M-Se			0.221
	M-D1			0.314
	M-D2			0.065
	M-D3			0.050
	M-D4			≤ 0.001
	D1-SD1			0.815
	D2-SD2			0.337
	D3-SD3			0.680
	D4-SD4			0.026

(c) Expression of DNMT3b in GPx1 knockdown splenic lymphocytes of pigs

DNMT3b mRNA
Time	Groups	Contents
48 h	P-M			0.053
	M-Se			0.152
	M-D1			0.013
	M-D2			0.074
	M-D3			0.010
	M-D4			0.021
	D1-SD1			0.388
	D2-SD2			0.554
	D3-SD3			0.043
	D4-SD4			0.174

(d) Expression of MBD2 in GPx1 knockdown splenic lymphocytes of pigs

MBD2 mRNA
Time	Groups	Contents
48 h	P-M			0.740
	M-Se			0.925
	M-D1			0.160
	M-D2			0.095
	M-D3			0.032
	M-D4			0.008
	D1-SD1			0.025
	D2-SD2			0.859
	D3-SD3			0.874
	D4-SD4			0.361