Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite

Table 5

(a) Activities of GSH-Px in GPx1 overexpressed splenic lymphocytes of pigs

GSH-Px
Time	Groups	Activities (U/mg·prot)
48 h	C-P			0.044
	P-K			0.500
	C-K			0.060
	C-SE			0.050
	C-D1			0.081
	C-D2			0.007
	C-D3			0.009
	C-D4			0.002
	D1-SD1			0.068
	D2-SD2			0.009
	D3-SD3			0.257
	D4-SD4			0.056

(b) Contents of GSH in GPx1-overexpressed splenic lymphocytes of pigs

GSH
Time	Groups	Contents (μmol/g·prot)
48 h	C-P			0.295
	P-K			0.583
	C-K			0.767
	C-SE			0.048
	C-D1			0.170
	C-D2			0.014
	C-D3			0.002
	C-D4			0.011
	D1-SD1			0.703
	D2-SD2			0.004
	D3-SD3			0.339
	D4-SD4			0.039

(c) Activities of SOD in GPx1-overexpressed splenic lymphocytes of pigs

SOD
Time	Groups	Activities (U/mg·prot)
48 h	C-P			0.165
	P-K			0.848
	C-K			0.387
	C-SE			0.130
	C-D1			0.057
	C-D2			0.094
	C-D3			0.008
	C-D4			0.024
	D1-SD1			0.010
	D2-SD2			0.078
	D3-SD3			0.133
	D4-SD4			0.003

(d) Activities of T-AOC in GPx1-overexpressed splenic lymphocytes of pigs

T-AOC
Time	Groups	Activities (U/mg·prot)
48 h	C-P			0.004
	P-K			0.959
	C-K			0.002
	C-SE			0.112
	C-D1			0.007
	C-D2			0.002
	C-D3			0.012
	C-D4			0.003
	D1-SD1			0.274
	D2-SD2			0.716
	D3-SD3			0.708
	D4-SD4			0.107

(e) Activities of CAT in GPx1-overexpressed splenic lymphocytes of pigs

CAT
Time	Groups	Activities (U/mg·prot)
48 h	C-P			0.035
	P-K			0.288
	C-K			0.013
	C-SE			0.005
	C-D1			0.001
	C-D2			0.001
	C-D3			0.002
	C-D4			0.001
	D1-SD1			0.404
	D2-SD2			0.058
	D3-SD3			0.121
	D4-SD4			0.037

(f) Contents of MDA in GPx1-overexpressing splenic lymphocytes of pigs

MDA
Time	Groups	Contents (nmol/mg·prot)
48 h	C-P			0.040
	P-K			0.905
	C-K			0.038
	C-SE			0.253
	C-D1			0.008
	C-D2			0.010
	C-D3			0.027
	C-D4			0.006
	D1-SD1			0.006
	D2-SD2			0.016
	D3-SD3			0.481
	D4-SD4			0.045

(g) Levels of Inhibition of hydroxyl radical in GPx1-overexpressed splenic lymphocytes of pigs

Inhibition of hydroxyl radical
Time	Groups
48 h	C-P			0.001
	P-K			0.001
	C-K			0.001
	C-SE			0.024
	C-D1			0.106
	C-D2			0.001
	C-D3			0.001
	C-D4			0.039
	D1-SD1			0.233
	D2-SD2			0.004
	D3-SD3			0.225
	D4-SD4			0.006

(h) Contents of H₂O₂ in GPx1-overexpressed splenic lymphocytes of pigs

H2O2
Time	Groups	Contents (mmol/g·prot)
48 h	C-P			0.142
	P-K			0.001
	C-K			0.085
	C-SE			0.742
	C-D1			0.001
	C-D2			0.001
	C-D3			0.002
	C-D4			0.001
	D1-SD1			0.092
	D2-SD2			0.008
	D3-SD3			0.032
	D4-SD4			0.002

(i) Levels of ROS in GPx1-overexpressed splenic lymphocytes of pigs

ROS
Time	Groups
48 h	C-P			0.064
	P-K			0.270
	C-K			0.644
	C-SE			0.003
	C-D1			0.005
	C-D2			0.009
	C-D3			0.005
	C-D4			0.052
	D1-SD1			0.372
	D2-SD2			0.196
	D3-SD3			0.013
	D4-SD4			0.159

(j) Expression of GPx1 in GPx1-overexpressed splenic lymphocytes of pigs

GPx1 mRNA
Time	Groups	Contents
48 h	C-P			0.378
	P-K			0.983
	C-K			0.100
	C-SE			0.003
	C-D1			0.753
	C-D2			0.695
	C-D3			0.069
	C-D4			0.040
	D1-SD1			≤0.001
	D2-SD2			0.004
	D3-SD3			0.004
	D4-SD4			0.030

(k) Expression of SOD in GPx1-overexpressed splenic lymphocytes of pigs

SOD mRNA
Time	Groups	Contents
48 h	C-P			0.518
	P-K			0.343
	C-K			0.888
	C-SE			0.015
	C-D1			0.010
	C-D2			0.007
	C-D3			0.010
	C-D4			0.011
	D1-SD1			0.039
	D2-SD2			0.009
	D3-SD3			0.010
	D4-SD4			0.041