Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite

Table 7

(a) Expression of DNMT1 in GPx1-overexpressed splenic lymphocytes of pigs

DNMT1 mRNA
Time	Groups	Contents
48 h	C-P			0.263
	P-K			0.115
	C-K			0.665
	C-SE			0.009
	C-D1			≤0.001
	C-D2			0.003
	C-D3			≤0.001
	C-D4			0.003
	D1-SD1			0.791
	D2-SD2			0.050
	D3-SD3			0.004
	D4-SD4			0.042

(b) Expression of DNMT3a in GPx1-overexpressed splenic lymphocytes of pigs

DNMT3a mRNA
Time	Groups	Contents
48 h	C-P			0.737
	P-K			0.069
	C-K			0.839
	C-SE			0.013
	C-D1			0.024
	C-D2			0.090
	C-D3			0.018
	C-D4			0.131
	D1-SD1			0.037
	D2-SD2			0.039
	D3-SD3			0.059
	D4-SD4			0.005

(c) Expression of DNMT3b in GPx1-overexpressed splenic lymphocytes of pigs

DNMT3b mRNA
Time	Groups	Contents
48 h	C-P			0.448
	P-K			0.609
	C-K			0.152
	C-SE			0.048
	C-D1			0.065
	C-D2			0.173
	C-D3			0.038
	C-D4			0.056
	D1-SD1			0.133
	D2-SD2			0.174
	D3-SD3			0.096
	D4-SD4			0.161

(d) Expression of MBD2 in GPx1-overexpressed splenic lymphocytes of pigs

MBD2 mRNA
Time	Groups	Contents
48 h	C-P			0.817
	P-K			0.824
	C-K			0.867
	C-SE			0.011
	C-D1			0.875
	C-D2			0.951
	C-D3			0.040
	C-D4			0.006
	D1-SD1			0.205
	D2-SD2			0.418
	D3-SD3			0.979
	D4-SD4			0.028