Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite

Table 8

(a) Activities of GSH-Px in GPx1 knockdown splenic lymphocytes of pigs

GSH-Px
Time	Groups	Activities (U/mg·prot)
48 h	P-M			0.063
	M-SE			0.557
	M-D1			0.122
	M-D2			0.228
	M-D3			0.121
	M-D4			0.019
	D1-SD1			0.672
	D2-SD2			0.967
	D3-SD3			0.951
	D4-SD4			0.932

(b) Contents of GSH in GPx1 knockdown splenic lymphocytes of pigs

GSH
Time	Groups	Contents (μmol/g·prot)
48 h	P-M			0.854
	M-SE			0.207
	M-D1			0.496
	M-D2			0.081
	M-D3			0.023
	M-D4			0.024
	D1-SD1			0.028
	D2-SD2			0.001
	D3-SD3			0.103
	D4-SD4			0.302

(c) Activities of SOD in GPx1 knockdown splenic lymphocytes of pigs

SOD
Time	Groups	Activities (U/mg·prot)
48 h	P-M			0.012
	M-SE			0.815
	M-D1			0.008
	M-D2			0.005
	M-D3			0.001
	M-D4			0.004
	D1-SD1			0.005
	D2-SD2			0.028
	D3-SD3			0.547
	D4-SD4			0.213

(d) Activities of T-AOC in GPx1 knockdown splenic lymphocytes of pigs

T-AOC
Time	Groups	Activities (U/mg·prot)
48 h	P-M			0.007
	M-SE			0.059
	M-D1			0.027
	M-D2			0.001
	M-D3			0.003
	M-D4			0.001
	D1-SD1			0.003
	D2-SD2			0.014
	D3-SD3			0.001
	D4-SD4			0.007

(e) Activities of CAT in GPx1 knockdown splenic lymphocytes of pigs

CAT
Time	Groups	Activities (U/mg·prot)
48 h	P-M			0.002
	M-SE			0.110
	M-D1			≤0.001
	M-D2			≤0.001
	M-D3			≤0.001
	M-D4			≤0.001
	D1-SD1			0.006
	D2-SD2			≤0.001
	D3-SD3			≤0.001
	D4-SD4			≤0.001

(f) Contents of MDA in GPx1 knockdown splenic lymphocytes of pigs

MDA
Time	Groups	Contents (nmol/mg·prot)
48 h	P-M			0.031
	M-SE			0.534
	M-D1			0.037
	M-D2			0.002
	M-D3			0.009
	M-D4			≤0.001
	D1-SD1			0.096
	D2-SD2			0.017
	D3-SD3			0.005
	D4-SD4			0.016

(g) Levels of Inhibition of hydroxyl radical in GPx1 knockdown splenic lymphocytes of pigs

Inhibition of hydroxyl radical
Time	Groups
48 h	P-M			0.731
	M-SE			0.827
	M-D1			0.423
	M-D2			0.776
	M-D3			0.423
	M-D4			0.778
	D1-SD1			0.423
	D2-SD2			0.769
	D3-SD3			0.733
	D4-SD4			0.778

(h) Contents of H₂O₂ in GPx1 knockdown splenic lymphocytes of pigs

H2O2
Time	Groups	Contents (mmol/g·prot)
48 h	P-M			0.013
	M-SE			0.007
	M-D1			0.008
	M-D2			0.001
	M-D3			0.002
	M-D4			≤0.001
	D1-SD1			0.005
	D2-SD2			0.014
	D3-SD3			0.029
	D4-SD4			0.064

(i) Levels of ROS in GPx1 knockdown splenic lymphocytes of pigs

ROS
Time	Groups
48 h	P-M			0.006
	M-SE			0.006
	M-D1			0.730
	M-D2			0.017
	M-D3			≤0.001
	M-D4			0.003
	D1-SD1			0.414
	D2-SD2			0.005
	D3-SD3			0.069
	D4-SD4			0.100

(j) Expression of GPx1 in GPx1 knockdown splenic lymphocytes of pigs

GPx1 mRNA
Time	Groups	Contents
48 h	P-M			0.564
	M-SE			0.445
	M-D1			0.989
	M-D2			0.238
	M-D3			0.008
	M-D4			0.052
	D1-SD1			0.225
	D2-SD2			≤0.001
	D3-SD3			0.064
	D4-SD4			0.724

(k) Expression of SOD in GPx1 knockdown splenic lymphocytes of pigs

SOD mRNA
Time	Groups	Contents
48 h	P-M			0.041
	M-SE			0.247
	M-D1			0.194
	M-D2			0.333
	M-D3			0.012
	M-D4			0.052
	D1-SD1			0.112
	D2-SD2			0.559
	D3-SD3			0.030
	D4-SD4			0.772