Internalization and Transportation of Endothelial Cell Surface K_Ca2.3 and K_Ca3.1 in Normal Pregnancy and Preeclampsia

Figure 5

Catalase and GPX1 knockdown slows down K_Ca2.3 or K_Ca3.1 endocytosis. (a) PM levels of K_Ca2.3 or K_Ca3.1 in MAECs from wild-type and catalase^-/-/GPX1^-/- mice. (b) Cav-1 levels in MAECs from wild-type and catalase^-/-/GPX1^-/- mice. (c) K_Ca2.3 and clathrin levels in MAECs from wild-type and catalase^-/-/GPX1^-/- mice. (d) Elevated K_Ca2.3 levels by catalase/GPX1 knockdown were reversed by a membrane permeable catalase, PEG-catalase. (e, f) A H₂O₂ donor TBHP reduced clathrin (e) or Rab5c (f) levels in a concentration-dependent manner. (g) K_Ca3.1 currents activated by loading cells with 1 μM Ca²⁺ (A) and the current-[Ca²⁺]_i relationship at +50 mV (B). The current densities at +50 mV are plotted against [Ca²⁺]_i. Blots shown are representative of three to four experiments performed with three to four different cultures. Bar graphs represent the . , .

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