Internalization and Transportation of Endothelial Cell Surface K<sub>Ca</sub>2.3 and K<sub>Ca</sub>3.1 in Normal Pregnancy and Preeclampsia

<div>Superoxide facilitates K<sub>Ca</sub>2.3 or K<sub>Ca</sub>3.1 endocytosis. (a) Elevated PM clathrin levels by PE serum were reduced by NOX4 inhibition using NOX4 siRNA. (b) Elevated Cav-1 levels by LPC were reduced by the antioxidants, tempol or tiron. (c) Elevated colocalization of K<sub>Ca</sub>3.1 with Cav-1, clathrin, or Rab5c by LPC was reversed by the treatment with a pan-NOX inhibitor, VAS2870, or apocynin. (d, e) A superoxide donor, X/XO, elevated Cav-1 (d) or Rab5c (e) levels in a concentration-dependent manner. Blots shown are representative of three to four experiments performed with three to four different cultures. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Internalization and Transportation of Endothelial Cell Surface K<sub>Ca</sub>2.3 and K<sub>Ca</sub>3.1 in Normal Pregnancy and Preeclampsia 

Oxidative Medicine and Cellular Longevity

Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia

Figure 6

Internalization and Transportation of Endothelial Cell Surface K_Ca2.3 and K_Ca3.1 in Normal Pregnancy and Preeclampsia