Higd1a Protects Cells from Lipotoxicity under High-Fat Exposure

Figure 7

PGC-1a and HIF-1a exert synergistic effects on inducing Higd1a expression. (a) LO2 cells were transfected with siRNA NC, siRNA PGC-1a, siRNA HIF-1a, or siRNA PGC-1a+siRNA HIF-1a and then treated with FFAs; the protein expression of PGC-1a, HIF-1a, and Higd1a was measured by western blot, and Higd1a expression was decreased in the FFA+siRNA PGC-1a+siRNA HIF-1a group compared with other groups. (b) LO2 cells were transfected with pcDNA NC, pcDNA PGC-1a, pcDNA HIF-1a, or pcDNA PGC-1a+pcDNA HIF-1a and then treated with FFAs, and western blot revealed that Higd1a expression was increased in the FFA+pcDNA PGC-1a+pcDNA HIF-1a group compared with other groups ( compared with the control group; compared with FFA+siRNA NC group or FFA+pcDNA NC group; compared with the FFA+siRNA PGC-1a group or FFA+pcDNA PGC-1a group; compared with the FFA+siRNA HIF-1a group or FFA+pcDNA HIF-1a group).

(a)

(b)