Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo

Figure 1

Effect of FE on the proliferation and cell cycle of human skin fibroblasts. (a) Human skin fibroblasts were treated with different concentrations of FE (0%, 1%, 5%, and 10%) for 72 h. Cell proliferation was quantified using the CCK-8 test. (b) The CCK-8 test showed cell proliferation at 72 h after cells were pretreated with or without FE (0%, 1%, 5%, and 10%) for 24 h and then exposed to UVB light. (c) Morphology of human skin fibroblasts at 72 h after UVB irradiation. Scale bar: 150 μm. (d) Cell cycle analysis was carried out 24 h postirradiation. Cell cycle distribution analyzed by flow cytometry. FE treatment significantly increased cell proliferation and abrogated UVB-induced cell cycle arrest ().

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