LncRNA AW112010 Promotes Mitochondrial Biogenesis and Hair Cell Survival: Implications for Age-Related Hearing Loss

Figure 3

LncRNA AW112010 promoted mitochondrial function and cell survival in HEI-OC1 cells. (a) HEI-OC1 cells were treated with 1 mM H₂O₂ for 6 h. RT-qPCR analysis showed that H₂O₂ treatment increased AW112010 expression in HEI-OC1 cells. (b) Serum deprivation elevated AW112010 expression in HEI-OC1 cells. (c) HEI-OC1 cells were incubated with 5 μM Compound C for 24 h. RT-qPCR analysis showed that Compound C decreased AW112010 expression in HEI-OC1 cells. (d) RT-qPCR analysis validation of AW112010 knockdown (si-AW112010) by Smart Silencer in HEI-OC1 cells. (e) ATP assay showed reduced ATP levels in AW112010 knockdown cells compared to negative control cells. (f) JC-1 assay revealed a decreased JC-1 polymer/monomer fluorescence ratio in AW112010 knockdown cells. (g) Mitochondrial ROS was measured using CM-H2XRos probes. Immunocytochemical analysis showed elevated red fluorescence in AW112010 knockdown cells under 1 mM H₂O₂ stimulation. Scale bar: 50 μm. (h) CCK-8 assay revealed decreased cell viability in AW112010 knockdown cells in the presence or absence of 1 mM H₂O₂ treatment compared to negative control cells. (i) RT-qPCR analysis validation of AW112010 overexpression (AW112010-OE) in HEI-OC1 cells. (j) CCK-8 assay showed increased cell viability in AW112010 overexpression cells under H₂O₂ treatment. , , and . Ctl: control; NC: negative control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)