LncRNA AW112010 Promotes Mitochondrial Biogenesis and Hair Cell Survival: Implications for Age-Related Hearing Loss

Figure 4

AW112010 induced mitochondrial biogenesis in HEI-OC1 cells. (a) Mitochondrial mass was measured using MitoTracker Red CMXRos probes. Mitochondrial staining showed reduced red fluorescence in AW112010 knockdown cells. Scale bar: 50 μm. (b) mtDNA (D-loop) levels were detected by qPCR and normalized to nuclear gene beta2-microglobulin. Silencing of AW112010 reduced the mtDNA content in HEI-OC1 cells. (c) Western blot and densitometry of mitochondrial biogenesis-related transcription factors PGC-1α and TFAM. Levels of PGC-1α and TFAM were normalized to the β-actin level. AW112010 knockdown reduced the expression of PGC-1α and TFAM in HEI-OC1 cells. (d) HEI-OC1 cells were preincubated with resveratrol (5 μM) for 24 h and then treated with H₂O₂ (1 mM) for 6 h. Western blot showed that resveratrol activated the expression of PGC-1α and TFAM in HEI-OC1 cells. (e) After AW112010 Smart Silencer transfection, cells were preincubated with resveratrol (5 μM) for 24 h and then treated with H₂O₂ (1 mM) for 2 h. CCK-8 assay revealed that resveratrol elevated cell viability against H₂O₂ insults in HEI-OC1 cells. (f) HEI-OC1 cells were incubated with 0.1 or 0.5 μM STR1720 for 24 h. Western blot showed that 0.5 μM SRT1720 induced the expression of PGC-1α and TFAM in HEI-OC1 cells. (g) After AW112010 Smart Silencer transfection, cells were preincubated with SRT1720 (0.5 μM) for 24 h and then treated with H₂O₂ (1 mM) for 2 h. CCK-8 assay revealed that SRT1720 increased cell viability against H₂O₂ insults in HEI-OC1 cells. , , and . RES: resveratrol; SRT: SRT1720.

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