Research Article
LncRNA AW112010 Promotes Mitochondrial Biogenesis and Hair Cell Survival: Implications for Age-Related Hearing Loss
Figure 7
Transcription factor Arid5b modulated the expression of AW112010 and mitochondrial biogenesis process. (a) RT-qPCR assay revealed a reduced expression of AW112010 in Arid5b knockdown cells compared to negative control cells. The expression of Arid5b in the mouse cochlea was measured by RT-qPCR (b) and Western blot assay (c). Elevated mRNA and protein expression of Arid5b was detected in old mice compared to young mice. RT-qPCR assay showed an increased expression of Arid5b in HEI-OC1 cells under the treatment of H2O2 (d) and serum deprivation (e) in HEI-OC1 cells. (f) Western blot assay showed that Arid5b knockdown inhibited the expression of PGC-1α and TFAM in HEI-OC1 cells. (g) CCK-8 assay revealed decreased cell viability in Arid5b knockdown cells in the presence or absence of 1 mM H2O2 treatment compared to negative control cells. , , and .
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