HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage

Figure 1

Effects of HB-EGF on the proliferation and differentiation of uterine stromal cells during in vitro decidualization. (a) Real-time PCR analysis of HB-EGF mRNA expression after treatment with estrogen and progesterone for 24, 36, 48, and 72 h. (b) ELISA analysis of HB-EGF protein after treatment with estrogen and progesterone. (c) Effects of HB-EGF on stromal cell proliferation. After treatment with rHB-EGF for 24 h in the presence of estrogen and progesterone, stromal cells were analyzed by MTS assay. (d and e) Flow cytometry analysis of HB-EGF role in cell cycle of stromal cells. (f) Effects of HB-EGF on the expression of Ccnd3 and Cdk4 in stromal cells. (g–i) Effects of HB-EGF on Prl8a2 and Prl3c1 expression as well as ALP activity. (j and k) Effects of HB-EGF siRNA on Prl8a2 and Prl3c1 expression as well as ALP activity. EP: estrogen plus progesterone; NC: negative control; siHB-EGF: HB-EGF siRNA. Data are shown as . Asterisks denote significance ().

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