HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage

Figure 3

HB-EGF protects stromal cell differentiation against H₂O₂-induced oxidative damage during in vitro decidualization. (a–c) NAC and HB-EGF abrogated the repression of H₂O₂ on Prl8a2 and Prl3c1 expression as well as ALP activity. After treatment with NAC or rHB-EGF and then addition of H₂O₂, Prl8a2 and Prl3c1 expression and ALP activity were determined in the presence of estrogen and progesterone. (d–f) NAC and HB-EGF weakened the induction of H₂O₂ on intracellular ROS level. After treatment with NAC or rHB-EGF and then addition of H₂O₂, intracellular ROS levels were detected by flow cytometry or Multi-Detection Microplate Reader in the presence of estrogen and progesterone. (g) NAC and HB-EGF impeded the induction of H₂O₂ on MDA content. (h and i) NAC blocked the induction of H₂O₂ on intracellular O₂⁻ level. Bars with different letters at the top differ significantly.

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