Modulation of O-GlcNAcylation Regulates Autophagy in Cortical Astrocytes

Figure 2

Inhibition of OGT by alloxan enhances autophagic flux in astrocytes. (a) Autophagic flux in astrocytes was examined by immunofluorescence staining with the astrocyte-specific markers anti-GFAP (red) and anti-LC3 (green) using a confocal microscope. CQ was treated before 2 h of fixation. (b) Counting and calculation of LC3 puncta formation (, ). (c) Immunoblotting of LC3 proteins was performed in cells treated with alloxan and CQ, and statistical analyses were performed by (, , , and ).

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