“Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers

Figure 4

(a) Dynamics of the EPR signal of mito-TEMPO (0.1 mM) in cells with different proliferative indexes (1 × 10⁶ cells/mL) within 6-hour incubation. Control: mito-TEMPO in cultured medium. Mito-TEMPO did not affect cell viability at the selected experimental conditions. NL: normal lymphocytes. (b) Integrated EPR signal of mito-TEMPO in cell suspensions after 6-hour incubation. (c) Proliferative index of cells. (d) Basic intracellular levels of superoxide, analyzed by a DHE test. (e) Basic intracellular levels of hydroperoxides, analyzed by a DCF test. The data in (d) and (e) were normalized to 1 × 10⁶ cells/mL. In (a), (b), (c), and (d), the data are means ± SD from six independent experiments. In (e), the data are means ± SD from nine independent experiments.

(a)

(b)

(c)

(d)

(e)