“Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers

Figure 7

(a) Doubling time of normal (FHC) and cancer (HT29, Colon26) colon epithelial cells. The doubling time of FHC was obtained in DMEM-F12 medium, supplemented with growth factors. (b) Glucose consumption in cells ( cells/24 hours). (c) EPR signal intensity of mito-TEMPO (0.1 mM) in cell suspensions ( cells/mL) within 6-hour incubation at 37°C. Control: mito-TEMPO (0.1 mM) in cultured medium. Mito-TEMPO did not affect cell viability at the selected experimental conditions. (d) Basal intracellular levels of antioxidants and reducing equivalents, analyzed by OxiSelect™ Total Antioxidant Capacity Assay. (e) Basal intracellular levels of superoxide, analyzed by a DHE test. (f) Basal intracellular levels of hydroperoxides, analyzed by a DCF test. The data in (c), (d), (e), and (f) were normalized to cells/mL. All data are the from three independent experiments. , , and versus FHC; and versus HT29; all other variations are insignificant.

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