Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway

Figure 3

Methane-rich saline inhibited the expression of hepatic fibrosis-associated protein in BDL rats. MRS treatment in rats was achieved by intraperitoneal administration (10 mL/kg) for 7 days after BDL. (a) Immunoblot assays of α-SMA, TGF-β1, and collagen I. Relative densities of (b) α-SMA, (c) TGF-β1, and (d) collagen I. (e) Immunohistochemical staining of α-SMA, TGF-β1, and collagen I. The percentage of (f–h) α-SMA, TGF-β1, and collagen I. (, scale bars: 20 μm. Data are shown as the . versus the sham control group; ^# versus the sham control group; ^€ versus the MRS control group; ^$ versus the MRS control group; ^& versus the BDL+NS group; ^γ versus the BDL+NS group).

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