Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway

Figure 5

Methane-rich saline downregulated the expression of the TLR/NF-κB signaling pathway. MRS treatment in rats was achieved by intraperitoneal administration (10 mL/kg) for 7 days after BDL. (a) Immunoblot assays of TLR4, Myd88, and NF-κB (p65). Relative densities of (b–d) TLR4, Myd88, and NF-κB (p65). (e) Immunofluorescence staining of TLR4. (f) The percentage of TLR4-positive cells. (g) Immunofluorescence staining of NF-κB (p65). (h) The percentage of NF-κB (p65)-positive cells. (, scale bars: 25 μm. Data are shown as the mean ± SD. versus the sham control group; ^# versus the sham control group; ^€ versus the MRS control group; ^$ versus the MRS control group; ^& versus the BDL+NS group; ^γ versus the BDL+NS group).

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