[Retracted] High Glucose Enhances Bupivacaine-Induced Neurotoxicity via MCU-Mediated Oxidative Stress in SH-SY5Y Cells

<div>Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mCa<sup>2+</sup> accumulation and mROS overproduction in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10 <i>μ</i>Μ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca<sup>2+</sup> was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa<sup>2+</sup> measured with flow cytometry. (b) Summarized data show mROS measured with flow cytometry. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: [Retracted] High Glucose Enhances Bupivacaine-Induced Neurotoxicity via MCU-Mediated Oxidative Stress in SH-SY5Y Cells 