Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation

Figure 2

Neutrophil infiltration attenuated by the neutrophil elastase inhibitor in injured lung post-OALT. Neutrophil infiltration was detected at 8 h post-OALT in the lung of the rat, which was given rSERPINB1 24 h or sivelestat sodium 1 h before surgery. Neutrophil infiltration was clearly observed via lung H&E ((a): 100x and 400x) staining and naphthol staining ((b) and (c): 200x). Moreover, the numbers of total cells (d), neutrophils (e), and macrophages (f) were counted in the collected BALF using Wright’s staining. NE activity (g) in BALF was determined via ELISA. Each bar represents the ( per group). and ; one-way ANOVA with Tukey’s test. rSERPINB1 (rSB1): recombinant SERPINB1 protein; Sit: sivelestat; BALF: bronchoalveolar lavage fluid; OALT: orthotopic autologous liver transplantation.

(a)

(b)

(c)

(d)

(e)

(f)

(g)