Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation

Figure 3

Attenuation of ALI, reduction of pulmonary fibrosis protein generation, and restoration of alveolar barrier function by neutrophil elastase inhibitor. Lung injury and lung tissue fibrosis-associated protein were evaluated using H&E staining ((a) and (b): 400x) and Masson’s staining ((c) and (d): 200x) at 8 h post-OALT in the rat lung which was given rSERPINB1 24 h or sivelestat sodium 1 h before surgery. Lung ZO-1 ((a) and (b): 200x) and occludin ((c) and (d): 200x) protein expressions were assayed by immunofluorescence at 8 h post-OALT in the rat lung which was given rSERPINB1 24 h or sivelestat sodium 1 h before surgery. Each bar represents the ( per group). and ; one-way ANOVA with Tukey test. rSERPINB1 (rSB1): SERPINB1 recombinant protein; Sit: sivelestat; OALT: orthotopic autologous liver transplantation.

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