Resumption of Autophagy by Ubisol-Q₁₀ in Presenilin-1 Mutated Fibroblasts and Transgenic AD Mice: Implications for Inhibition of Senescence and Neuroprotection

Figure 1

(a) Autophagy-related gene expression profile for Normal Human Fibroblasts (NHF), untreated PSAF, PTS-treated PSAF, and Ubisol-Q10-treated PSAF. NHF cells served as a positive control whereas untreated and PTS-treated AD cells served as negative controls. Notably, autophagy-related genes beclin-1, MAPK8/JNK1, and CTSB were upregulated in AD cells treated with Ubisol-Q10 bringing expression levels similar to or higher than NHF. (b) Beclin-1 probing of whole cell lysates via Western blot from NHF and PSAF. Supporting gene analysis, Beclin-1 was upregulated in Ubisol-Q10-treated PSAF and not untreated or PTS-treated AD cells. (c, d) Immunofluorescence staining of NHF and PSAF probing for beclin-1 (green) and JNK1 (green) and quantification of fluorescence, respectively. Treatment of PSAF cells with Ubisol-Q10 led to increased staining for beclin-1 and JNK1 compared to the untreated and PTS-treated groups indicating upregulation of autophagic proteins. These Ubisol-Q10-treated PSAF were stained in a comparable manner to NHF cells. Nuclei were counterstained with Hoechst for visualization. Micrographs were taken at 200x magnification. Scale bar = 50 μm.

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